Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22841-78] to MRP1 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, Flow Cyt (Intra)
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free
    See all MRP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22841-78] to MRP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt (Intra)more details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, HepG2, K-562, Human lung cancer, Human lung, NIH/3T3, Mouse liver and Mouse stomach lysates. IHC-P: Human breast carcinoma, Human gastric adenocarcinoma, Human lung carcinoma and the paired adjacent lung and Mouse stomach tissues. Flow Cyt (intra): A549 and NIH/3T3 cells. IP: A549 and NIH/3T3 cells.

  • General notes

    ab263865 is the carrier-free version of ab260038.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)
    Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)

    MRP1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab260038 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10ug

    Lane 2: ab260038 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate, boiled 10ug

    Lane 3: NIH/3T3 whole cell lysate

    Lane 4: Rabbit monoclonal IgG (ab172730) instead of ab260038 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 75 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

  • Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)
    Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)

    MRP1 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate  with ab260038 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab260038 IP in A549 (human lung carcinoma epithelial cell) whole cell lysate, boiled 10ug

    Lane 3: A549 whole cell lysate

    Lane 4: Rabbit monoclonal IgG (ab172730) instead of ab260038 in A549 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on mouse stomach (PMID: 23667609). The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma and the paired adjacent lung tissue tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on human lung carcinoma (panel A) and no staining on the paired adjacent lung tissue (panel B, PMID: 25880778). The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)

    Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on human gastric adenocarcinoma. The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on human breast carcinoma (PMID: 15517899). The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

  • Flow Cytometry - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)
    Flow Cytometry - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling MRP1 with ab260038 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

  • Flow Cytometry - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)
    Flow Cytometry - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell, Left) / A549 (human lung carcinoma epithelial cell, Right) cells labelling MRP1 with ab260038 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HEK-293 (PMID: 29615646，PMID:12235150).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

  • Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)
    Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free (ab263865)

© 2021 Astana Biomed Group LLP. All rights reserved.