Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)
![Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)](https://www.abcam.com/ps/products/180/ab180960/Images/ab180960-352598-anti-mrp1-antibody-epr46582-western-blot-knockout-hela-human-lysates.jpg "Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4658(2)] to MRP1 - C-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MRP1 antibody [EPR4658(2)] - C-terminal
See all MRP1 primary antibodies -
Description
Rabbit monoclonal [EPR4658(2)] to MRP1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A431: HeLa and A549 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4658(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)All lanes : Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/1000 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : ABCC1 knockout A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 171 kDaLanes 1 - 2: Merged signal (red and green). Green - ab180960 observed at 170 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab180960 was shown to specifically react with ABCC1 in wild-type A549 cells as signal was lost in ABCC1 knockout cells. Wild-type and ABCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab180960 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging
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![Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)](https://www.abcam.com/ps/products/180/ab180960/Images/ab180960-338635-anti-mrp1-antibody-epr46582-c-terminal-western-blot.png)
Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)All lanes : Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (boiled)
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 171 kDa
Exposure time: 20 secondsBlocking and dilution buffer: 5% NFDM/TBST.
If there is no ideal result on boiled sample, we suggest not to boil sample before loading onto the gel.
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![Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)](https://www.abcam.com/ps/products/180/ab180960/Images/ab180960-212267-anti-mrp1-antibody-epr46582-c-terminal-western-blot.jpg)
Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/5000 dilution + A549 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 171 kDa
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![Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)](https://www.abcam.com/ps/products/180/ab180960/Images/ab180960-6-benefits-of-recombinant-antibodies.png)
Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)
