Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MRP2 antibody [EPR10998] (ab172630)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10998] to MRP2
Suitable for: WB, ICC/IF, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-MRP2 antibody [EPR10998]
See all MRP2 primary antibodies
Description

Rabbit monoclonal [EPR10998] to MRP2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MRP2 aa 1500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q92887
Positive control
- WB: Human fetal liver, HepG2 and A549 lysates. ICC/IF: HepG2 cells and A549 cells. Flow Cyt: Permeabilized A549 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10998
Isotype

IgG
Research areas

Western blot - Anti-MRP2 antibody [EPR10998] (ab172630)

All lanes : Anti-MRP2 antibody [EPR10998] (ab172630) at 1/1000 dilution

Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : ABCC2 knockout A549 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : HeLa whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 174 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab172630 observed at 210 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab172630 was shown to recognize MRP2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab172630 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-MRP2 antibody [EPR10998] (ab172630)

Anti-MRP2 antibody [EPR10998] (ab172630) at 1/2000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 174 kDa
Observed band size: >190 kDa
why is the actual band size different from the predicted?

The multiple bands are consistent with what has been described in PMID: 18832477
Immunocytochemistry/ Immunofluorescence - Anti-MRP2 antibody [EPR10998] (ab172630)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling MRP2 with purified ab172630 at 1:100 dilution (7.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-MRP2 antibody [EPR10998] (ab172630)

Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling MRP2 with purified ab172630 at 1:70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-MRP2 antibody [EPR10998] (ab172630)

All lanes : Anti-MRP2 antibody [EPR10998] (ab172630) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : A549 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 174 kDa
Flow Cytometry - Anti-MRP2 antibody [EPR10998] (ab172630)

Flow cytometric analysis of permeabilized A549 cells using ab172630 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).
Immunocytochemistry/ Immunofluorescence - Anti-MRP2 antibody [EPR10998] (ab172630)

Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling MRP2 with ab172630 at a 1/250 dilution.
Anti-MRP2 antibody [EPR10998] (ab172630)

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