Anti-SLC12A1/NKCC2 antibody [EPR11842] (ab171747)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11842] to SLC12A1/NKCC2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-SLC12A1/NKCC2 antibody [EPR11842]
See all SLC12A1/NKCC2 primary antibodies -
Description
Rabbit monoclonal [EPR11842] to SLC12A1/NKCC2 -
Host species
Rabbit -
Specificity
The homology between SLC12A1/NKCC2 and SLC12A2 is 67%, so ab171747 may cross react with SLC12A2. We haven't performed any laboratory testing about this.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
Recombinant fragment within Human SLC12A1/NKCC2. The exact sequence is proprietary.
Database link: Q13621 -
Positive control
- 293T, Human fetal kidney, K562 and WI-38 cell lysates; permeabilized K562 cells.
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General notes
This product was previously labelled as SLC12A1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11842 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SLC12A1/NKCC2 antibody [EPR11842] (ab171747) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : Human fetal kidney lysates
Lane 3 : K562 cell lysates
Lane 4 : WI-38 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 121 kDa
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Flow cytometric analysis of permeabilized K562 cells labeling SLC12A1/NKCC2 with ab171747 at 1/100 dilution (red), compared with a rabbit IgG (negative) (green).
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