Anti-SLC12A1/NKCC2 antibody (ab91098)
Key features and details
- Rabbit polyclonal to SLC12A1/NKCC2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLC12A1/NKCC2 antibody
See all SLC12A1/NKCC2 primary antibodies -
Description
Rabbit polyclonal to SLC12A1/NKCC2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human SLC12A1/NKCC2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101074) -
Positive control
- This antibody gave a positive signal in Human kidney tissue lysate.
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General notes
This product was previously labelled as SLC12A1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRabbitOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).Target
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Function
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. -
Tissue specificity
Kidney specific. -
Involvement in disease
Defects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia. -
Sequence similarities
Belongs to the SLC12A transporter family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6557 Human
- Entrez Gene: 20495 Mouse
- Entrez Gene: 100328575 Rabbit
- Omim: 600839 Human
- SwissProt: Q13621 Human
- SwissProt: P55014 Mouse
- SwissProt: P55015 Rabbit
- Unigene: 123116 Human
see all -
Alternative names
- BSC1 antibody
- Bumetanide sensitive sodium 3 antibody
- Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody
see all
Images
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Western blot - Anti-SLC12A1/NKCC2 antibody (ab91098)Anti-SLC12A1/NKCC2 antibody (ab91098) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesSLC12A1/NKCC2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab91098 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-SLC12A1/NKCC2 antibody (ab91098)Anti-SLC12A1/NKCC2 antibody (ab91098) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesSLC12A1/NKCC2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
