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Signal Transduction Metabolism Plasma Membrane Channels

Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (ab213653)

Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (ab213653)
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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11842] to SLC12A1/NKCC2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free
    See all SLC12A1/NKCC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11842] to SLC12A1/NKCC2 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The homology between SLC12A1/NKCC2 and SLC12A2 is 67%, so ab213653 may cross react with SLC12A2. We have not performed any laboratory testing to validate this.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human SLC12A1/NKCC2. The exact sequence is proprietary.
    Database link: Q13621

  • Positive control

    • WB: 293T, Human fetal kidney, K562 and WI-38 whole cell lysates. Flow Cyt: K562 cells.
  • General notes

    ab213653 is the carrier-free version of ab171747.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11842
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels

Images

  • Western blot - Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (ab213653)
    Western blot - Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (ab213653)
    All lanes : Anti-SLC12A1/NKCC2 antibody [EPR11842] (ab171747) at 1/1000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : Human fetal kidney lysates
    Lane 3 : K562 cell lysates
    Lane 4 : WI-38 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 121 kDa



    This data was developed using ab171747, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (ab213653)
    Flow Cytometry - Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (ab213653)
    This data was developed using ab171747, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized K562 cells labeling SLC12A1/NKCC2 with ab171747 at 1/100 dilution (red), compared with a rabbit IgG (negative) (green).
  • Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (ab213653)
    Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (ab213653)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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