Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
![Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)](https://www.abcam.com/ps/products/264/ab264038/Images/ab264038-1-anti-myosin-myh3-antibody-f1652-immunohistochemistry-mouse-e145.jpg "Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [F1.652] to heavy chain Myosin/MYH3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-heavy chain Myosin/MYH3 antibody [F1.652]
See all heavy chain Myosin/MYH3 primary antibodies -
Description
Mouse monoclonal [F1.652] to heavy chain Myosin/MYH3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatWB MouseRat
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Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse embryo tissue lysate; L6 whole cell lysate; Mouse 14.5 limb tissue lysate. IHC-P: Mouse 14.5 tissue; Rat 14.5 tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
F1.652 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
Immunohistochemical analysis of paraffin-embedded mouse E14.5 tissue labeling Myosin (MYH3) with ab264038 at 1/500 dilution (2.22 µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of mouse E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)](https://www.abcam.com/ps/products/264/ab264038/Images/ab264038-395318-anti-myosin-myh3-antibody-f1652-western-blot-mouse-embryo-tissue-lysate-20ug.jpg)
Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)All lanes : Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038) at 0.222 µg/ml
Lane 1 : Mouse embryo tissue lysate
Lane 2 : Mouse adult skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 224 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Negative control: Mouse adult skelatal muscle (PMID:3530499).
The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)](https://www.abcam.com/ps/products/264/ab264038/Images/ab264038-2-anti-myosin-myh3-antibody-f1652-immunohistochemistry-rat-e145.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)Immunohistochemical analysis of paraffin-embedded rat E14.5 tissue labeling Myosin (MYH3) with ab264038 at 1/500 dilution (2.22 µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of rat E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)](https://www.abcam.com/ps/products/264/ab264038/Images/ab264038-395317-anti-myosin-myh3-antibody-f1652-western-blot-l6.jpg)
Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)All lanes : Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038) at 0.222 µg/ml
Lane 1 : L6 (rat skeletal muscle myoblast), whole cell lysate
Lane 2 : Mouse E14.5 limb tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 224 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730).
-
![Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)](https://www.abcam.com/ps/products/264/ab264038/Images/ab264038-5-benefits-of-recombinant-antibodies.png)
Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
