Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [F1.652] to heavy chain Myosin/MYH3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-heavy chain Myosin/MYH3 antibody [F1.652]
    See all heavy chain Myosin/MYH3 primary antibodies

  • Description

    Mouse monoclonal [F1.652] to heavy chain Myosin/MYH3

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse embryo tissue lysate; L6 whole cell lysate; Mouse 14.5 limb tissue lysate. IHC-P: Mouse 14.5 tissue; Rat 14.5 tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    F1.652

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 tissue labeling Myosin (MYH3) with ab264038 at 1/500 dilution (2.22 µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of mouse E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
    Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
    All lanes : Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038) at 0.222 µg/ml

    Lane 1 : Mouse embryo tissue lysate
    Lane 2 : Mouse adult skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 224 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: Mouse adult skelatal muscle (PMID:3530499).


    The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)

    Immunohistochemical analysis of paraffin-embedded rat E14.5 tissue labeling Myosin (MYH3) with ab264038 at 1/500 dilution (2.22 µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of rat E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
    Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
    All lanes : Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038) at 0.222 µg/ml

    Lane 1 : L6 (rat skeletal muscle myoblast), whole cell lysate
    Lane 2 : Mouse E14.5 limb tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 224 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730).

  • Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
    Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)

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