Anti-Fox2 / RBM9 antibody (ab264154)
Key features and details
- Rabbit polyclonal to Fox2 / RBM9
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Fox2 / RBM9 antibody
See all Fox2 / RBM9 primary antibodies -
Description
Rabbit polyclonal to Fox2 / RBM9 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide aa 1-50. The exact sequence is proprietary. NP_055124.1
Database link: O43251 -
Positive control
- WB: HeLa, HEK-203T, NIH/3T3, TCMK, 4T1, CT26 and C6 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human lung carcinoma and mouse renal cell carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Fox2 / RBM9 antibody (ab264154)All lanes : Anti-Fox2 / RBM9 antibody (ab264154) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 41 kDa
Exposure time: 3 minutes4-20% SDS-PAGE
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Western blot - Anti-Fox2 / RBM9 antibody (ab264154)All lanes : Anti-Fox2 / RBM9 antibody (ab264154) at 0.5 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : TCMK (Mouse kidney epithelial cell line) whole cell lysate
Lane 3 : 4T1 (Mouse mammary gland carcinoma cell line) whole cell lysate
Lane 4 : CT26 (Mouse colon carcinoma fibroblast cell line) whole cell lysate
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 41 kDa
Exposure time: 3 minutes4-20% SDS-PAGE
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Immunoprecipitation - Anti-Fox2 / RBM9 antibody (ab264154)Fox2 / RBM9 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264154 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264154 at 1 µg/ml.
Lane 1: ab264154 IP in HeLa whole cell lysate.
Lane 2: ab264154 IP in HeLa whole cell lysate.
Lane 3: Control IgG.
Exposure time: 30 secs.
Different lots used in lane 1 and lane 2.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fox2 / RBM9 antibody (ab264154)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma (left panel) and mouse renal cell carcinoma (right panel) labeling Fox2 / RBM9 with ab264154 at 1/1,000 dilution. Detection: DAB.
