Anti-Dynamin 2 antibody (ab227192)
Key features and details
- Rabbit polyclonal to Dynamin 2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Dynamin 2 antibody
See all Dynamin 2 primary antibodies -
Description
Rabbit polyclonal to Dynamin 2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Xenopus laevis, Rhesus monkey -
Immunogen
Recombinant fragment within Human Dynamin 2 (internal sequence). The exact sequence is proprietary.
Database link: P50570 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: PC-12, HeLa and Rat2 whole cell extracts. Rat and mouse brain tissue extracts. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Dynamin 2 antibody (ab227192) at 1/5000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 98 kDa7.5% SDS PAGE
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Methanol fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Dynamin 2 (green) using ab227192(1/500 dilution) in ICC/IF.
Counterstain: Hoechst 33342 (blue).
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Paraffin embedded human breast carcinoma tissue stained for Dynamin 2 with ab227192 (1/500 dilution) in immunohistochemical analysis.
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Anti-Dynamin 2 antibody (ab227192) at 1/5000 dilution + Mouse brain tissue extracts at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 98 kDa7.5% SDS-PAGE
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Anti-Dynamin 2 antibody (ab227192) at 1/5000 dilution + Rat brain tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 98 kDa7.5% SDS-PAGE
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All lanes : Anti-Dynamin 2 antibody (ab227192) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extracts
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 98 kDa7.5% SDS-PAGE