Anti-Gliomedin antibody (ab24483)
Key features and details
- Rabbit polyclonal to Gliomedin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Gliomedin antibody -
Description
Rabbit polyclonal to Gliomedin -
Host species
Rabbit -
Specificity
ab24483 detects a Gliomedin sized band at 70kDa in WB. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatWB MouseRat
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Immunogen
Synthetic peptide corresponding to Rat Gliomedin aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab25746) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Sciatic Nerve (Rat and Mouse) IF/ICC: PC12 cell line
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF RatWB MouseRatAll applications HumanApplication Abreviews Notes WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).ICC/IF Use a concentration of 10 µg/ml.Notes WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).ICC/IF
Use a concentration of 10 µg/ml.Target
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Relevance
Gliomedin is a member of the collagen superfamily, it is a glial ligand for neurofascin and NrCAM, two axonal immunoglobulin cell adhesion molecules that are associated with Na+ channels at the nodes of Ranvier. Gliomedin provides a glial cue for the formation of peripheral nodes of Ranvier. Gliomedin is expressed by myelinating Schwann cells and accumulates at the edges of each myelin segment during development, where it aligns with the forming nodes of ranvier. Eliminating the expression of gliomedin or the addition of a soluble extracellular domain of neurofascin to myelinating cultures abolishes node formation. Gliomedin is expressed in the PNS nodes of ranvier, but not in the CNS nodes of ranvier. Gliomedin also displays high expression in murine and human hepatocellular carcinomas (HCC). Its restricted expression in normal tissues and unique early upregulation during tumor development make it an excellent candidate as a new clinical marker of HCC. -
Cellular localization
Cell Membrane and Cytoplasmic -
Database links
- Entrez Gene: 342035 Human
- Entrez Gene: 235379 Mouse
- Entrez Gene: 315675 Rat
- Omim: 608603 Human
- SwissProt: Q6ZMI3 Human
- SwissProt: Q8BMF8 Mouse
- SwissProt: Q80WL1 Rat
- Unigene: 526441 Human
see all -
Alternative names
- CANCER RELATED GENE LIVER 2 antibody
- Cancer related gene-Liver 2 antibody
- CLOM antibody
see all
Images
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Western blot - Anti-Gliomedin antibody (ab24483)All lanes : Anti-Gliomedin antibody (ab24483) at 1 µg/ml
Lane 1 : Mouse Sciatic Nerve Tissue Lysate
Lane 2 : Rat Sciatic Nerve Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesab24483 detects a band of 70 kDa in Western Blot. Gliomedin contains a number of N-glycosylation sites (SwissProt data) which may explain the migration at a higher molecular weight than predicted based on primary sequence.
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Immunocytochemistry/ Immunofluorescence - Anti-Gliomedin antibody (ab24483)ICC/IF image of ab24483 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24483, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (4)
ab24483 has been referenced in 4 publications.
- Kwon HS et al. Myocilin mediates myelination in the peripheral nervous system through ErbB2/3 signaling. J Biol Chem 288:26357-71 (2013). PubMed: 23897819
- Kawamura N et al. Anti-neurofascin antibody in patients with combined central and peripheral demyelination. Neurology 81:714-22 (2013). Human . PubMed: 23884033
- Thaxton C et al. Nodes of Ranvier Act as Barriers to Restrict Invasion of Flanking Paranodal Domains in Myelinated Axons. Neuron 69:244-57 (2011). PubMed: 21262464
- Thaxton C et al. In vivo deletion of immunoglobulin domains 5 and 6 in neurofascin (Nfasc) reveals domain-specific requirements in myelinated axons. J Neurosci 30:4868-76 (2010). PubMed: 20371806
Images
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Western blot - Anti-Gliomedin antibody (ab24483)All lanes : Anti-Gliomedin antibody (ab24483) at 1 µg/ml
Lane 1 : Mouse Sciatic Nerve Tissue Lysate
Lane 2 : Rat Sciatic Nerve Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesab24483 detects a band of 70 kDa in Western Blot. Gliomedin contains a number of N-glycosylation sites (SwissProt data) which may explain the migration at a higher molecular weight than predicted based on primary sequence.
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Immunocytochemistry/ Immunofluorescence - Anti-Gliomedin antibody (ab24483)
ICC/IF image of ab24483 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24483, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.