PACSIN1 overexpression 293T lysate (whole cell) (ab94237)
Overview
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Product name
PACSIN1 overexpression 293T lysate (whole cell) -
General notes
ab94237 is a 293T cell transfected lysate in which Human PACSIN1 has been transiently over-expressed using a pCMV-PACSIN1 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: May play a role in vesicle formation and transport. Tissue specificity: Highly expressed in brain and in a lesser extent in heart, pancreas and liver. Similarity: Belongs to the PACSIN family. Contains 1 FCH domain. Contains 1 SH3 domain. PTM: Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
Images
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SDS-PAGE - PACSIN1 overexpression 293T lysate (whole cell) (ab94237)ab94237 at 15µg/lane on an SDS-PAGE gel.
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Western blot - PACSIN1 overexpression 293T lysate (whole cell) (ab94237)All lanes : Anti-PACSIN1 antibody (ab89604) at 1/500 dilution
Lane 1 :PACSIN1 overexpression 293T lysate (whole cell) (ab94237)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution