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Anti-Rad21 antibody (ab154769)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Rad21 antibody (ab154769)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Rad21
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Rad21 antibody
    See all Rad21 primary antibodies
  • Description

    Rabbit polyclonal to Rad21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 6-399 of Human Rad21 (UniProt ID: O60216).

  • Positive control

    • K562, THP1, HL60, NCI-H929, NIH3T3, JC and BCL-1 whole cell lysate; HeLa cells; Human HBL435 and Mouse C2C12 xenograft tissues
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Cohesins
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion
    • Cancer
    • Cell cycle
    • Cell division

Images

  • Western blot - Anti-Rad21 antibody (ab154769)
    Western blot - Anti-Rad21 antibody (ab154769)
    All lanes : Anti-Rad21 antibody (ab154769) at 1/1000 dilution

    Lane 1 : K562 whole cell lysate
    Lane 2 : THP1 whole cell lysate
    Lane 3 : HL60 whole cell lysate
    Lane 4 : NCI-H929 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 72 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (ab154769)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (ab154769)
    Immunohistochemical analysis of paraffin embedded Human HBL435 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-Rad21 antibody (ab154769)
    Immunocytochemistry/ Immunofluorescence - Anti-Rad21 antibody (ab154769)
    Immunofluorescent analysis of paraformaldehyde fixed HeLa cells labeling Rad21 with ab154769 at 1/500 dilution. Lower image shows cells costained with Hoechst 33342.
  • Western blot - Anti-Rad21 antibody (ab154769)
    Western blot - Anti-Rad21 antibody (ab154769)
    All lanes : Anti-Rad21 antibody (ab154769) at 1/3000 dilution

    Lane 1 : NIH3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 72 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (ab154769)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (ab154769)
    Immunohistochemical analysis of paraffin embedded Mouse C2C12 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Rad21 antibody (ab154769)

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    Anti-Rad21 antibody [EPR12647] - BSA and Azide free (ab241395)

    Applications: Flow Cyt, WB

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    Anti-Rad21 antibody [52A311] (ab153765)

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    Anti-Rad21 antibody [EPR12647] (ab175215)

    Applications: Flow Cyt, WB

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    Anti-Rad21 antibody (ab992)

    Applications: WB

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    Anti-Rad21 antibody [BLR052F] (ab243905)

    Applications: WB

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    Anti-Rad21 antibody (ab171462)

    Applications: WB

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