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Anti-Rad21 antibody [EPR12647] (ab175215)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Rad21 antibody [EPR12647] (ab175215)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12647] to Rad21
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-Rad21 antibody [EPR12647]
    See all Rad21 primary antibodies
  • Description

    Rabbit monoclonal [EPR12647] to Rad21
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Rad21 aa 600 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O60216

  • Positive control

    • THP-1, 293T, K562 and HeLa whole cell lysate (ab150035); K562 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12647
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Cohesins
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion
    • Cancer
    • Cell cycle
    • Cell division

Images

  • Flow Cytometry - Anti-Rad21 antibody [EPR12647] (ab175215)
    Flow Cytometry - Anti-Rad21 antibody [EPR12647] (ab175215)

    Flow cytometric analysis of permeabilized K562 cells labeling Rad21 with ab175215 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Western blot - Anti-Rad21 antibody [EPR12647] (ab175215)
    Western blot - Anti-Rad21 antibody [EPR12647] (ab175215)
    All lanes : Anti-Rad21 antibody [EPR12647] (ab175215) at 1/1000 dilution

    Lane 1 : THP-1 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Hela cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 72 kDa

  • Anti-Rad21 antibody [EPR12647] (ab175215)
    Anti-Rad21 antibody [EPR12647] (ab175215)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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