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Human Serpin A12 ELISA Kit (ab239430)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.12 pg/ml
  • Range: 19.53 pg/ml - 1250 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Serpin A12 ELISA Kit
    See all Vaspin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 2.1%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 4.3%

  • Sample type

    Saliva, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3.12 pg/ml

  • Range

    19.53 pg/ml - 1250 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 97 87% - 105%
    Serum 107 103% - 110%
    Cell culture media 97 96% - 100%
    Hep Plasma 100 97% - 105%
    EDTA Plasma 99 95% - 102%
    Cit plasma 89 81% - 102%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Serpin A12 ELISA Kit (ab239430) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Serpin A12 protein in cit plasma, edta plasma, hep plasma, saliva, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Serpin A12 with 3.12 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human Serpin A12 ELISA Kit (ab239430)
    Other - Human Serpin A12 ELISA Kit (ab239430)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Human Serpin A12 gamma Standard Curve Comparison
    Human Serpin A12 gamma Standard Curve Comparison
    Standard Curve comparison between Human Serpin A12 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
  • Example of human Serpin A12 standard curve in Sample Diluent NS.
    Example of human Serpin A12 standard curve in Sample Diluent NS.
    The Serpin A12 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • Interpolated concentrations of native Serpin A12 in human serum, plasma and saliva samples.
    Interpolated concentrations of native Serpin A12 in human serum, plasma and saliva samples.
    The concentrations of Serpin A12 were measured in duplicates, interpolated from the Serpin A12 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 15%, plasma (citrate) 50%, plasma (EDTA) 100%, plasma (heparin) 25% and saliva 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serpin A12 concentration was determined to be 4862.24 pg/mL in serum, 1422.93 pg/mL in plasma (citrate), 1275.97 pg/mL in plasma (EDTA), 4493.91 pg/mL in plasma (heparin), and 208.10 pg/mL in saliva.
  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serpin A12 concentration was determined to be 5068.08 pg/mL with a range of undetectable - 9406.87 pg/mL.

  • Serum from ten individual healthy human female donors was measured in duplicate.
    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serpin A12 concentration was determined to be 1672.82 pg/mL with a range of undetectable - 6317.42 pg/mL.

  • Sandwich ELISA - Human Serpin A12 ELISA Kit (ab239430)
    Sandwich ELISA - Human Serpin A12 ELISA Kit (ab239430)
    To learn more about the advantages of recombinant antibodies see here.

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