Human Insulin like Growth Factor 1 ELISA Kit (IGF1) (ab108873)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.4 ng/ml - 24 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Insulin like Growth Factor 1 ELISA Kit (IGF1)
See all IGF1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.4% Inter-assay Sample n Mean SD CV% Overall 8.9% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.4 ng/ml -
Range
0.4 ng/ml - 24 ng/ml -
Recovery
= 98 %
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Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Insulin like Growth Factor 1 (IGF1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Insulin like Growth Factor 1 concentrations in Human cell culture supernatants, serum and plasma.
An Insulin like Growth Factor 1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Insulin like Growth Factor 1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Insulin like Growth Factor 1 captured in plate.
Get results in 90 minutes with Human IGF1 ELISA Kit (ab211651) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Plasma/Serum Pretreatment Buffer 1 x 7.5ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Insulin like Growth Factor 1 Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Insulin like Growth Factor 1 Microplate (12 x 8 well strips) 1 unit Insulin like Growth Factor 1 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IBP1
- IGF I
- IGF IA
see all -
Database links
- Entrez Gene: 3479 Human
- Omim: 147440 Human
- SwissProt: P05019 Human
- Unigene: 160562 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108873.
