Human IGF1 ELISA Kit (ab100545)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.1 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml Assay Diluent C 2 x 30ml Biotinylated anti-Human IGF-1 (lyophilized) 2 vials IGF-1 Microplate (12 strips x 8 wells) 1 unit Recombinant Human IGF1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IBP1
- IGF I
- IGF IA
see all -
Database links
- Entrez Gene: 3479 Human
- Omim: 147440 Human
- SwissProt: P05019 Human
- Unigene: 160562 Human
Images
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Typical Standard Curve
Representative standard curve using ab100545.
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Sandwich ELISA - IGF1 Human ELISA Kit (ab100545)IGF1 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-64 fold.
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Sandwich ELISA - IGF1 Human ELISA Kit (ab100545)Human IGF1 standard curve: mean of duplicates (+/- SD)
