Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDX1 antibody [EPR3358(2)] (ab134150)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3358(2)] to PDX1
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PDX1 antibody [EPR3358(2)]
    See all PDX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3358(2)] to PDX1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PDX1 (C terminal). The exact sequence is proprietary.
    Database link: P52945

  • Positive control

    • CACO-2 and Beta-TC-6 cell lysates, Human pancreas & duodenum tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    Western blot - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    All lanes : Anti-PDX1 antibody [EPR3358(2)] (ab134150) at 1/1000 dilution

    Lane 1 : adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 30 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    The expression profile observed in BxPC-3 is consistent with the literature (PMID: 12947327). Negative control:   BxPC-3 (PMID: 12947327)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

    ab134150 staining PDX1 in Human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Immunocytochemistry - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    Immunocytochemistry - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

    ab134150 staining PDX1 in the BXPC-3 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde. Samples were incubated with primary antibody (1/150). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG (1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

    Immunohistochemical analysis of paraffin embedded Human pancreas tissue labelled with ab134150, unpurified, at 1/1000 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

    Immunohistochemical analysis of paraffin embedded Human duodenum tissue labelled with ab134150, unpurified, at 1/1000 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150) This image is courtesy of an anonymous Abreview

    ab134150, unpurified, staining PDX1 in Human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in 10mM sodium citrate, pH 6.0. Samples were incubated with primary antibody (1/1000 in 2% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

    Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab134150, unpurified, showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

    Immunohistochemical analysis of paraffin embedded Human Heart muscles tissue using ab134150, unpurified, showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

    Immunohistochemical analysis of paraffin embedded normal Human Normal brain tissue using ab134150, unpurified, showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    OI-RD Scanning - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PDX1 antibody [EPR3358(2)] (ab134150)
    Anti-PDX1 antibody [EPR3358(2)] (ab134150)

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