Anti-PDX1 antibody (ab227586)
Key features and details
- Rabbit polyclonal to PDX1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PDX1 antibody
See all PDX1 primary antibodies -
Description
Rabbit polyclonal to PDX1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Dog, Pig
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Immunogen
Synthetic peptide within Human PDX1 (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P52945 -
Positive control
- WB: Jurkat, Raji, NCI-H929 and AsPC-1 whole cell extracts; Mouse liver and pancreas liver tissue extracts; Rat liver tissue extract. IHC-P: Rat pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab227586)
Paraffin-embedded rat pancreas tissue stained for PDX1 using ab227586 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-PDX1 antibody (ab227586)All lanes : Anti-PDX1 antibody (ab227586) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 31 kDa12% SDS-PAGE gel.
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Western blot - Anti-PDX1 antibody (ab227586)Anti-PDX1 antibody (ab227586) at 1/1000 dilution + Mouse pancreas tissue extract at 50 µg
Predicted band size: 31 kDa12% SDS-PAGE gel.
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Western blot - Anti-PDX1 antibody (ab227586)Anti-PDX1 antibody (ab227586) at 1/1000 dilution + AsPC-1 whole cell extract at 30 µg
Predicted band size: 31 kDa12% SDS-PAGE gel.
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Western blot - Anti-PDX1 antibody (ab227586)Anti-PDX1 antibody (ab227586) at 1/1000 dilution + Rat liver tissue extract at 50 µg
Predicted band size: 31 kDa12% SDS-PAGE gel.
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Western blot - Anti-PDX1 antibody (ab227586)Anti-PDX1 antibody (ab227586) at 1/1000 dilution + Mouse liver tissue extract at 50 µg
Predicted band size: 31 kDa12% SDS-PAGE gel.
