Anti-PDX1 antibody [OTI2A12] (ab84987)
![Anti-PDX1 antibody [OTI2A12] (ab84987)](https://www.abcam.com/ps/products/84/ab84987/Images/ab84987-99795-anti-pdx1-antibody-oti2a12-immunohistochemistry.jpg "Anti-PDX1 antibody [OTI2A12] (ab84987)")
Key features and details
- Mouse monoclonal [OTI2A12] to PDX1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PDX1 antibody [OTI2A12]
See all PDX1 primary antibodies -
Description
Mouse monoclonal [OTI2A12] to PDX1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-283 of human PDX1 expressed in E. coli.
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Positive control
- IHC-P: pancreas; carcinoma of pancreas WB: HEK293T cells transfected with PDX1; lysate from 293T cells transfected with PDX1 ICC/IF: HeLa cells transiently transfected by PDX1; HeLa cells
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General notes
The clone number has been updated from 2A12 to OTI2A12, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI2A12 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987)ab84987, staining PDX1 in paraffin embedded human pancreas carcinoma tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987)](https://www.abcam.com/ps/products/84/ab84987/Images/ab84987-99794-anti-pdx1-antibody-oti2a12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987)ab84987, staining PDX1 in paraffin embedded human pancreas by Immunohistochemistry. -
![Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)](https://www.abcam.com/ps/products/84/ab84987/Images/ab84987-99793-anti-pdx1-antibody-oti2a12-western-blot.jpg)
Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)Anti-PDX1 antibody [OTI2A12] (ab84987) at 1/20000 dilution + lysate from HEK293T cells transfected with human PDX1 expression vector
Predicted band size: 31 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033) -
![Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)](https://www.abcam.com/ps/products/84/ab84987/Images/ab84987-99792-anti-pdx1-antibody-oti2a12-western-blot.jpg)
Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)All lanes : Anti-PDX1 antibody [OTI2A12] (ab84987)
Lane 1 : lysate from HEK293T cells transfected with an empty expression vector
Lane 2 : lysate from HEK293T cells transfected with a human PDX1 expression vector
Lysates/proteins at 5 µg per lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033) -
![Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [OTI2A12] (ab84987)](https://www.abcam.com/ps/products/84/ab84987/Images/ab84987-99791-anti-pdx1-antibody-oti2a12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [OTI2A12] (ab84987)ab84987, staining PDX1 by Immunofluoresence in HeLa cells transiently transfected with a vector expressing PDX1.
