Human IGF1 ELISA Kit (ab211651)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.97 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IGF1 ELISA Kit
See all IGF1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.9% Inter-assay Sample n Mean SD CV% Serum 3 9.5% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
11.97 pg/ml -
Range
93.75 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 86 83% - 90% Cell culture media 100 96% - 104% Hep Plasma 100 91% - 111% EDTA Plasma 113 106% - 118% Cit plasma 100 97% - 104% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IGF1 ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human IGF1 ELISA Kit (ab211651) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGF1 protein in serum, cell culture media, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IGF1 with 11.97 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY This kit recognizes both native and recombinant human IGF1 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.
This kit is incompatible with cell culture supernatant samples than contain bovine serum.
CROSS REACTIVITY Recombinant human IGF2 and IGFBP3 were prepared at 16000 and 8000 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE Recombinant IGF1 and IGFBP3 were prepared at 12000 pg/mL and 12000 p/mL and tested for interference. No interference with was observed.
SPECIES REACTIVITY This kit recognizes human IGF1 protein. Other species reactivity was determined by measuring 1.25% plasma - citrate samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human plasma - citrate assayed at the same dilution. Reactive species: Bovine, Primate. Reactivity -
Notes
Insulin-like growth factor I (IGF1) is a 70 amino acid hormone that this proteolytically processed from a larger precursor protein. IGF1 is structurally similar to insulin and has growth promoting effects.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IGF1 Capture Antibody 1 x 600µl 10X Human IGF1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 4BR 1 x 6ml Denaturant 1 x 500µl Human IGF1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IBP1
- IGF I
- IGF IA
see all -
Database links
- Entrez Gene: 3479 Human
- Omim: 147440 Human
- SwissProt: P05019 Human
- Unigene: 160562 Human
Images
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Other - Human IGF1 ELISA Kit (ab211651)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IGF1 competitor curve comparisonStandard Curve comparison between human IGF1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human IGF1 standard curve comparisonStandard Curve comparison between the original human IGF1 SimpleStep ELISA kit and current human IGF1 SimpleStep ELISA kit.
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Example of human IGF1 standard curve in Sample Diluent NS + 1X Enhancer.The IGF1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IGF1 standard curve in Sample Diluent NS + 1X Enhancer.Example of human IGF1 standard curve in Sample Diluent NS + 1X Enhancer. The IGF1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IGF1 in human serum and plasma samples.The concentrations of IGF1 were measured in duplicates, interpolated from the IGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 1.25%, plasma (EDTA) 1.25%, and plasma (heparin) 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 concentration was determined to be 99.58 ng/mL in serum, 121.23 pg/mL in plasma (citrate), 103.65 ng/mL in plasma (EDTA), and 110.43 ng/mL in plasma (heparin).
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1% Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 concentration was determined to be 78.06 pg/mL with a range of 45.41 – 141.07 pg/mL.
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1% Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 concentration was determined to be 129.12 ng/mL with a range of 69.09-184.22 ng/mL.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native IGF1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
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Linearity of dilution.Recombinant IGF1 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer.
