Anti-Desmoglein 3/PVA antibody (ab78448)
Key features and details
- Rabbit polyclonal to Desmoglein 3/PVA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Desmoglein 3/PVA antibody
See all Desmoglein 3/PVA primary antibodies -
Description
Rabbit polyclonal to Desmoglein 3/PVA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Desmoglein 3/PVA aa 850-950 conjugated to keyhole limpet haemocyanin.
(Peptide available asab87441) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; A431.
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General notes
Previously labelled as Desmoglein 3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).Target
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Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
Tissue specificity
Epidermis, tongue, tonsil, esophagus and carcinomas. -
Sequence similarities
Contains 4 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. Cell junction > desmosome. - Information by UniProt
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Database links
- Entrez Gene: 1830 Human
- Omim: 169615 Human
- SwissProt: P32926 Human
- Unigene: 1925 Human
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Alternative names
- 130 kD pemphigus vulgaris antigen antibody
- 130 kDa pemphigus vulgaris antigen antibody
- Balding antibody
see all
Images
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Western blot - Anti-Desmoglein 3/PVA antibody (ab78448)All lanes : Anti-Desmoglein 3/PVA antibody (ab78448) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References (0)
ab78448 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Desmoglein 3/PVA antibody (ab78448)All lanes : Anti-Desmoglein 3/PVA antibody (ab78448) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
