Cdc25C overexpression 293T lysate (whole cell) (ab94219)
Overview
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Product name
Cdc25C overexpression 293T lysate (whole cell)
See all Cdc25C lysates -
General notes
ab94219 is a 293T cell transfected lysate in which Human Cdc25C has been transiently over-expressed using a pCMV-Cdc25C plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Developmental stage: Expressed predominantly in G2 phase. Function: Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity. PTM: Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Similarity: Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.
Images
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SDS-PAGE - Cdc25C overexpression 293T lysate (whole cell) (ab94219)ab94219 at 15μg/lane on an SDS-PAGE gel.
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Western blot - Cdc25C overexpression 293T lysate (whole cell) (ab94219)All lanes : Anti-Cdc25C antibody (ab66235) at 1/500 dilution
Lane 1 : Cdc25C 293T Transfected Lysate - Positive Control (ab94219)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
