Anti-LMX1A antibody - C-terminal (ab139726)
Key features and details
- Rabbit polyclonal to LMX1A - C-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LMX1A antibody - C-terminal
See all LMX1A primary antibodies -
Description
Rabbit polyclonal to LMX1A - C-terminal -
Host species
Rabbit -
Specificity
At least two isoforms are known to exist. ab139726 is predicted to not cross-react with LMX1B. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanWB Rat
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Immunogen
Synthetic peptide within Human LMX1A aa 300-350 (C terminal). The exact sequence is proprietary. 16 amino acids. NP_796372
Database link: NP_796372 -
Positive control
- WB: Rat brain tissue lysate. IHC-P: Mouse and human brain tissue. ICC/IF: Mouse neural stem cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-LMX1A antibody - C-terminal (ab139726) This image is courtesy of an Abreview submitted by Sébastien Brot
ab139726 staining LMX1A (red) in mouse neural stem cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PBS + 3% BSA + 0.3% Triton X-100) for 16 hours at 4°C. An Alexa-Fluor® 568-conjugated donkey anti-rabbit IgG (H+L) monoclonal was used as the secondary antibody.
Blue - DAPI, Green - Nestin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody - C-terminal (ab139726)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling LMX1A with ab139726 at 5 μg/ml.
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Western blot - Anti-LMX1A antibody - C-terminal (ab139726)Lane 1 : Anti-LMX1A antibody - C-terminal (ab139726) at 1 µg
Lane 2 : Anti-LMX1A antibody - C-terminal (ab139726) at 2 µg
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 42 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody - C-terminal (ab139726)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human brain tissue labeling LMX1A with antibody ab139726 at 2.5 μg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-LMX1A antibody - C-terminal (ab139726)Immunofluorescence of human brain tissue labeling LMX1A with ab139726 at 20 μg/ml.
