Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-AMF antibody [EPR11663(B)] (ab167394)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11663(B)] to AMF
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-AMF antibody [EPR11663(B)]
See all AMF primary antibodies
Description

Rabbit monoclonal [EPR11663(B)] to AMF
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human AMF.
Database link: P06744
Positive control
- WB: HEK-293T, HepG2, HeLa, HT29, and A549 cell lysates. IHC-P: Human brain, pancreas, skeletal muscle, ovarian carcinoma, prostatic hyperplasia and thyroid carcinoma tissues. ICC/IF: MCF7 and HepG2 cells. Flow Cyt: A549 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as Glucose 6 phosphate isomerase

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11663(B)
Isotype

IgG
Research areas

Western blot - Anti-AMF antibody [EPR11663(B)] (ab167394)

All lanes : Anti-AMF antibody [EPR11663(B)] (ab167394) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : GPI knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 63 kDa
Observed band size: 63 kDa

Lanes 1-4: Merged signal (red and green). Green - ab167394 observed at 63 kDa. Red - loading control ab8245 observed at 36 kDa.

ab167394 Anti-AMF antibody [EPR11663(B)] was shown to specifically react with AMF in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266834 (knockout cell lysate ab257458) was used. Wild-type and AMF knockout samples were subjected to SDS-PAGE. ab167394 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-AMF antibody [EPR11663(B)] (ab167394)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling AFM with purified ab167394 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

Control: PBS only
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)

ab167394 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-AMF antibody [EPR11663(B)] (ab167394)

Flow cytometric analysis of permeabilized A549 cells, labeling AFM with ab167394 at 1/10 dilution.
Western blot - Anti-AMF antibody [EPR11663(B)] (ab167394)

All lanes : Anti-AMF antibody [EPR11663(B)] (ab167394) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT29 cell lysate
Lane 4 : A549 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 63 kDa
Immunocytochemistry/ Immunofluorescence - Anti-AMF antibody [EPR11663(B)] (ab167394)

Immunofluorescent analysis of HepG2 cells labeling AFM with ab167394 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)

ab167394 showing +ve staining in Human normal pancreas.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)

ab167394 showing +ve staining in Human ovarian carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)

ab167394 showing +ve staining in Human skeletal muscle.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue, labeling AFM with ab167394 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling AFM with ab167394 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-AMF antibody [EPR11663(B)] (ab167394)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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