Human LOX1 Matched Antibody Pair Kit (ab216063)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 7.81 pg/ml - 500 pg/ml
Overview
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Product name
Human LOX1 Matched Antibody Pair Kit
See all LOX 1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
5.09 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human LOX1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human LOX1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab212161. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human LOX1 Capture Antibody 2 x 50µg 1 x 50µg Human LOX1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human LOX1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. -
Tissue specificity
Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. -
Involvement in disease
Note=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. -
Sequence similarities
Contains 1 C-type lectin domain. -
Domain
The cytoplasmic region is required for subcellular sorting on the cell surface.
The C-type lectin domain mediates the recognition and binding of oxLDL. -
Post-translational
modificationsThe intrachain disulfide-bonds prevent N-glycosylation at some sites.
N-glycosylated. -
Cellular localization
Cell membrane. Secreted. A secreted form also exists. - Information by UniProt
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Alternative names
- C-type lectin domain family 8 member A
- CLEC8A
- hLOX 1
see all -
Database links
- Entrez Gene: 4973 Human
- Omim: 602601 Human
- SwissProt: P78380 Human
- Unigene: 412484 Human
Images
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Human LOX1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human LOX1 Matched Antibody Pair Kit (ab216063)To learn more about the advantages of recombinant antibodies see here.
