Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-PD-L1 antibody [BLR020E] (ab243877)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR020E] to PD-L1
Suitable for: WB, ICC/IF, IHC-P, IP
Reacts with: Human

Product name

Anti-PD-L1 antibody [BLR020E]
See all PD-L1 primary antibodies
Description

Rabbit monoclonal [BLR020E] to PD-L1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Mouse
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human PD-L1 aa 250-290 (C terminal). The exact sequence is proprietary. NP_054862.1 and Gene ID 29126.
Database link: Q9NZQ7
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR020E
Isotype

IgG
Research areas

Immunoprecipitation - Anti-PD-L1 antibody [BLR020E] (ab243877)

PDL-1 was immunoprecipitated from SUP-M2 whole cell lysate with ab243877. Western blot was performed on the immunoprecipitate using ab243877 at 1/1000 dilution.
Lane 1: ab243877 IP in SUP-M2 whole cell lysate.

Lane 2: Contol IgG in SUP-M2 whole cell lysate.

Detection: Chemiluminescence.
Western blot - Anti-PD-L1 antibody [BLR020E] (ab243877)

Western blot analysis using ab243877 at 1/1000 dilution.
Lane 1: SUP-M2 whole cell lysate (50 µg).
Lane 2: HDLM-2 whole cell lysate (50 µg).
Lane 3: HEK-293T whole cell lysate (50 µg).
Lane 4: Ramos whole cell lysate (50 µg).
Lane 5: Jurkat whole cell lysate (50 µg).
Lane 6: PD-L2 whole cell lysate (50 µg).
Lane 7: PD-L1 whole cell lysate (50 µg).
Lane 8: HEK-293 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence.
Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [BLR020E] (ab243877)

Formaldehyde-fixed HDML-2 cells labeling PDL-1 using ab243876 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (ab243877)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD-L1 using ab243877 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (ab243877)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PD-L1 using ab243877 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [BLR020E] (ab243877)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PD-L1 using ab243877 at 1/100 dilution in immunohistochemical analysis. A DyLight𪨂-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Anti-PD-L1 antibody [BLR020E] (ab243877)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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