Anti-p95/NBS1 antibody (ab227068)
Key features and details
- Rabbit polyclonal to p95/NBS1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p95/NBS1 antibody
See all p95/NBS1 primary antibodies -
Description
Rabbit polyclonal to p95/NBS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment within Human p95/NBS1 (internal sequence). The exact sequence is proprietary.
Database link: O60934 -
Positive control
- WB: HEK-293T, A431, HeLa, HepG2 and NTERA-2 cl.D1 [NT2/D1] whole cell extracts. IP: NTERA-2 cl.D1 [NT2/D1] whole cell extract. ICC/IF: A431 cells.
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General notes
This product was previously labelled as p95 NBS1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-p95/NBS1 antibody (ab227068) at 1/2000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T, transfected with p95/NBS1 shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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p95/NBS1 was immunoprecipitated from NTERA-2 cl.D1 [NT2/D1] (humanmalignant pluripotent embryonic carcinoma cell line) whole cell extract with 4 µg ab227068. Western blot was performed from the immunoprecipitate using ab227068 at 1/500 dilution.
Lane 1: NTERA-2 cl.D1 [NT2/D1] whole cell extract 50 µg.
Lane 2: Control IP in NTERA-2 cl.D1 [NT2/D1] whole cell extract with 4 μg of preimmune Rabbit IgG.
Lane 3: ab227068 IP in NTERA-2 cl.D1 [NT2/D1] whole cell extract.
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4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for p95/NBS1 (green) using ab227068 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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All lanes : Anti-p95/NBS1 antibody (ab227068) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Anti-p95/NBS1 antibody (ab227068) at 1/5000 dilution + NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 85 kDa7.5 % SDS-PAGE gel.