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Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)

Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15505] to TSPEAR - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-TSPEAR antibody [EPR15505] - BSA and Azide free
    See all TSPEAR primary antibodies
  • Description

    Rabbit monoclonal [EPR15505] to TSPEAR - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251105 is the carrier-free version of ab192023. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251105 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR15505
  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)

    This data was developed using ab192023, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    All lanes : Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100 dilution

    Predicted band size: 75 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab192023, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/5000 dilution + human testis tissue lysate at 1/10 dilution

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 75 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    This data was developed using ab192023, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    All lanes : Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 75 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    This data was developed using ab192023, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    This data was developed using ab192023, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    Immunocytochemistry - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    This data was developed using ab192023, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling TSPEAR using ab192023 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain: ab7291 and ab150120 both at 1/500 dilution. The negative controls: Primary ab concentration (anti-TSPEAR) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution. Cells were permeabilized using 0.1% Triton X-100.
  • Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)
    Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (ab251105)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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