Human IL-17F ELISA Set (without plates) (ab105233)
Overview
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Product name
Human IL-17F ELISA Set (without plates)
See all IL-17F kits -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Abcam’s IL-17F Human in vitro ELISA Set is designed for the quantitative measurement of IL-17F in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
ab105233 includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
ab105233 is intended to measure human IL-17F in cell culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The assay recognizes natural and recombinant human IL-17A.
RANGE: 15.6pg/ml - 500pg/ml
SENSITIVITY: -
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests Detection biotinylated anti-IL-17F antibody 1 vial 2 vials IL-17F Capture Antibody 1 x 0.5ml 2 x 0.5ml IL-17F Standard 5 vials 10 vials Ready-to-use TMB 2 vials 4 vials Streptavidin-HRP 1 vial 2 vials -
Research areas
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Function
Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation. -
Tissue specificity
Expressed in activated, but not resting, CD4+ T-cells and activated monocytes. -
Involvement in disease
Candidiasis, familial, 6 -
Sequence similarities
Belongs to the IL-17 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CANDF6
- Cytokine ML 1
- Cytokine ML-1
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Images
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IL-17F Human ELISA Set (ab105233)
Figure 2: IL-17F Standard curve ranging from 15.6 to 500 pg/mL obtained using ab105233