Anti-p95/NBS1 (phospho S343) antibody (ab47272)
Key features and details
- Rabbit polyclonal to p95/NBS1 (phospho S343)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p95/NBS1 (phospho S343) antibody
See all p95/NBS1 primary antibodies -
Description
Rabbit polyclonal to p95/NBS1 (phospho S343) -
Host species
Rabbit -
Specificity
Detects endogenous levels of p95/NBS1 only when phosphorylated at Serine 343.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human p95/NBS1.
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Positive control
- Jurkat cell extract.
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General notes
This product was previously labelled as p95 NBS1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against the non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p95/NBS1 (phospho S343) antibody (ab47272)All lanes : Anti-p95/NBS1 (phospho S343) antibody (ab47272) at 1/500 dilution
Lane 1 : 5-30ug Jurkat cell extract, treated with immunogenic peptide.
Lane 2 : 5-30ug Jurkat cell extract, untreated.
Secondary
All lanes : Alkaline Phosphatase Goat Anti IgG (H+L)
Predicted band size: 85 kDa
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Western blot - Anti-p95/NBS1 (phospho S343) antibody (ab47272) This image is courtesy of an anonymous AbreviewAll lanes : Anti-p95/NBS1 (phospho S343) antibody (ab47272) at 1/500 dilution
Lane 1 : Unirradiated Human lymphoblastoid cell lines from normal individual - Whole cell lysate
Lane 2 : Irradiated Human lymphoblastoid cell lines from normal individual - Whole cell lysate
Lane 3 : Unirradiated Human lymphoblastoid cell lines from classical A-T patient (no ATM kinase expressed) - Whole cell lysate
Lane 4 : Irradiated Human lymphoblastoid cell lines from classical A-T patient (no ATM kinase expressed) - Whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat polyclonal to rabbit IgG at 1/3000 dilution
Predicted band size: 85 kDa
