Anti-CHST3 antibody [EPR15789] (ab193359)
![Anti-CHST3 antibody [EPR15789] (ab193359)](https://www.abcam.com/ps/products/193/ab193359/Images/ab193359-230929-anti-chst3-antibody-epr15789-western-blot.jpg "Anti-CHST3 antibody [EPR15789] (ab193359)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15789] to CHST3
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-CHST3 antibody [EPR15789]
See all CHST3 primary antibodies -
Description
Rabbit monoclonal [EPR15789] to CHST3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB MouseHuman
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293, HeLa, HepG2, Jurkat and Raw264.7 cell lysates; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15789 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CHST3 antibody [EPR15789] (ab193359)All lanes : Anti-CHST3 antibody [EPR15789] (ab193359) at 1/1000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CHST3 antibody [EPR15789] (ab193359)](https://www.abcam.com/ps/products/193/ab193359/Images/ab193359-230928-anti-chst3-antibody-epr15789-western-blot.jpg)
Western blot - Anti-CHST3 antibody [EPR15789] (ab193359)Anti-CHST3 antibody [EPR15789] (ab193359) at 1/1000 dilution + Raw264.7 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry - Anti-CHST3 antibody [EPR15789] (ab193359)](https://www.abcam.com/ps/products/193/ab193359/Images/ab193359-230927-anti-chst3-antibody-epr15789-flow-cytometry.jpg)
Flow Cytometry - Anti-CHST3 antibody [EPR15789] (ab193359)Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling CHST3 with ab193359 at 1/350 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Anti-CHST3 antibody [EPR15789] (ab193359)](https://www.abcam.com/ps/products/193/ab193359/Images/ab193359-4-benefits-of-recombinant-antibodies.png)
Anti-CHST3 antibody [EPR15789] (ab193359)
