Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LILRB3 antibody [FRAS92B] (ab271287)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [FRAS92B] to LILRB3
  • Suitable for: Flow Cyt, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-LILRB3 antibody [FRAS92B]
    See all LILRB3 primary antibodies

  • Description

    Rat monoclonal [FRAS92B] to LILRB3

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human non-hodgkin's lymphoma and tonsil tissue. ICC: 293T cells. Flow Cyt: 293T cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    FRAS92B

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] (ab271287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] (ab271287)

    Immunohistochemical analysis of paraffin-embedded human non-hodgkin's lymphoma tissue labeling LILRB3 with ab271287 at 1.762µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human non-hodgkin's lymphoma. The section was incubated with ab271287 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] (ab271287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] (ab271287)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LILRB3 with ab271287 at 1.762µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human tonsil. The section was incubated with ab271287 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-LILRB3 antibody [FRAS92B] (ab271287)
    Immunocytochemistry - Anti-LILRB3 antibody [FRAS92B] (ab271287)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T cells labelling LILRB3 with ab271287 at 3.524µg/ml , followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
    Confocal image showing membranous and cytoplasmic staining on 293T cells transfected with LILRB3-myc plasmid.
    Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain Myc at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-LILRB3 antibody [FRAS92B] (ab271287)
    Flow Cytometry - Anti-LILRB3 antibody [FRAS92B] (ab271287)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with myc tagged LILRB3 construct cells labelling LILRB3 with ab271287 at 0.881µg/ml  (Right panel) compared with a rat monoclonal IgG isotype control (Left panel). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

  • Anti-LILRB3 antibody [FRAS92B] (ab271287)
    Anti-LILRB3 antibody [FRAS92B] (ab271287)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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