Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2588(2)] to SOCS2
  • Suitable for: ICC, WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-SOCS2 antibody [EPR2588(2)]
    See all SOCS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2588(2)] to SOCS2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NCCIT and K-562 whole cell lysates; IP: K-562 whole cell lysate; Flow Cyt: HeLa cells; ICC: MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    Western blot - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    All lanes : Anti-SOCS2 antibody [EPR2588(2)] (ab109245) at 1/2000 dilution (purified)

    Lane 1 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunocytochemistry - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    Immunocytochemistry - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling SOCS2 with purified ab109245 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    Flow Cytometry - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SOCS2 with purified ab109245 at 1/150 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    Immunoprecipitation - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

    Purified ab109245 at 1/70 dilution (2 µg) immunoprecipitating SOCS2 in K-562 whole cell lysate.
    Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg
    Lane 2 (+): ab109245 + K-562 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109245 in K-562 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 22 kDa

  • Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

© 2021 Astana Biomed Group LLP. All rights reserved.