Anti-MHF2 antibody (ab104236)
Key features and details
- Rabbit polyclonal to MHF2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MHF2 antibody -
Description
Rabbit polyclonal to MHF2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human MHF2 aa 1 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab157687) -
Positive control
- This antibody gave a positive signal in Human Liver tissue lysate as well as the following human whole cell lysates: HEK293; U2OS; HepG2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications ChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 9 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 9 kDa).Target
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Function
DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation. -
Sequence similarities
Belongs to the CENPX family. -
Cellular localization
Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 201254 Human
- SwissProt: A8MT69 Human
- Unigene: 37616 Human
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Alternative names
- CENP X antibody
- CENP-X antibody
- CENPX antibody
see all
Images
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Western blot - Anti-MHF2 antibody (ab104236)All lanes : Anti-MHF2 antibody (ab104236) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 46 kDa, 51 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab104236 detects a band at 11 kDa. While this differs to its predicted molecular weight of 9 kDa, the expression profile observed is consistent with what has been described in the literature PMID:20347429. This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab104236 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab104236 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-MHF2 antibody (ab104236)All lanes : Anti-MHF2 antibody (ab104236) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 46 kDa, 51 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab104236 detects a band at 11 kDa. While this differs to its predicted molecular weight of 9 kDa, the expression profile observed is consistent with what has been described in the literature PMID:20347429. This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab104236 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
