Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µg 274 732 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Rabbit polyclonal to SOCS2
Suitable for: WB
Reacts with: Mouse, Human
Isotype: IgG

Product name

Anti-SOCS2 antibody
See all SOCS2 primary antibodies
Description

Rabbit polyclonal to SOCS2
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human SOCS2 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab66732)
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HepG2, HeLa, SW480, Raji, MOLT4 and in Mouse Thymus tissue lysate

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Batches of this product that have a concentration
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
Recombinant Protein
- Recombinant Human SOCS2 protein (ab131812)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab66733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
WB	Human
All applications	Rat Cow Pig Chimpanzee

Application	Abreviews	Notes
WB		Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Function

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificity

High expression in heart, placenta, lung, kidney and prostate.
Pathway

Protein modification; protein ubiquitination.
Sequence similarities

Contains 1 SH2 domain.
Contains 1 SOCS box domain.
Domain

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
Target information above from: UniProt accession O14508 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 338437 Cow
- Entrez Gene: 8835 Human
- Entrez Gene: 216233 Mouse
- Entrez Gene: 84607 Rat
- Omim: 605117 Human
- SwissProt: Q861R0 Cow
- SwissProt: O14508 Human
- SwissProt: O35717 Mouse
- SwissProt: Q7YRV6 Pig
- SwissProt: O88582 Rat
- Unigene: 485572 Human
- Unigene: 474283 Mouse
- Unigene: 205056 Rat
see all
Alternative names
- CIS 2 antibody
- CIS-2 antibody
- CIS2 antibody
- Cish 2 antibody
- Cish2 antibody
- Cytokine inducible SH2 protein 2 antibody
- Cytokine-inducible SH2 protein 2 antibody
- SOCS 2 antibody
- SOCS-2 antibody
- Socs2 antibody
- SOCS2_HUMAN antibody
- SSI 2 antibody
- SSI-2 antibody
- SSI2 antibody
- STAT induced STAT inhibitor 2 antibody
- STAT-induced STAT inhibitor 2 antibody
- STATI 2 antibody
- STATI2 antibody
- Suppressor of cytokine signaling 2 antibody
see all

Western blot - Anti-SOCS2 antibody (ab66733)

All lanes : Anti-SOCS2 antibody (ab66733) at 1 µg/ml

Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 whole cell lysate (ab3957)
Lane 4 : Raji whole cell lysate (ab30124)
Lane 5 : Mouse thymus tissue lysate - total protein (ab29285)
Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Western blot protocols

Click here to view the general protocols

Publishing research using ab66733? Please let us know so that we can cite the reference in this datasheet.

ab66733 has not yet been referenced specifically in any publications.

Western blot - Anti-SOCS2 antibody (ab66733)

All lanes : Anti-SOCS2 antibody (ab66733) at 1 µg/ml

Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 whole cell lysate (ab3957)
Lane 4 : Raji whole cell lysate (ab30124)
Lane 5 : Mouse thymus tissue lysate - total protein (ab29285)
Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size: 22 kDa
Observed band size: 22 kDa