Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOCS2 antibody (ab66733)

Key features and details

  • Rabbit polyclonal to SOCS2
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SOCS2 antibody
    See all SOCS2 primary antibodies

  • Description

    Rabbit polyclonal to SOCS2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human SOCS2 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab66732)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HepG2, HeLa, SW480, Raji, MOLT4 and in Mouse Thymus tissue lysate

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab66733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Rat
Cow
Pig
Chimpanzee
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

  • Function

    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

  • Tissue specificity

    High expression in heart, placenta, lung, kidney and prostate.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.

  • Domain

    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CIS 2 antibody
    • CIS-2 antibody
    • CIS2 antibody
    • Cish 2 antibody
    • Cish2 antibody
    • Cytokine inducible SH2 protein 2 antibody
    • Cytokine-inducible SH2 protein 2 antibody
    • SOCS 2 antibody
    • SOCS-2 antibody
    • Socs2 antibody
    • SOCS2_HUMAN antibody
    • SSI 2 antibody
    • SSI-2 antibody
    • SSI2 antibody
    • STAT induced STAT inhibitor 2 antibody
    • STAT-induced STAT inhibitor 2 antibody
    • STATI 2 antibody
    • STATI2 antibody
    • Suppressor of cytokine signaling 2 antibody
    see all

Images

  • Western blot - Anti-SOCS2 antibody (ab66733)
    Western blot - Anti-SOCS2 antibody (ab66733)
    All lanes : Anti-SOCS2 antibody (ab66733) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 whole cell lysate (ab3957)
    Lane 4 : Raji whole cell lysate (ab30124)
    Lane 5 : Mouse thymus tissue lysate - total protein (ab29285)
    Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

References (0)

Publishing research using ab66733? Please let us know so that we can cite the reference in this datasheet.

ab66733 has not yet been referenced specifically in any publications.

Images

  • Western blot - Anti-SOCS2 antibody (ab66733)
    Western blot - Anti-SOCS2 antibody (ab66733)
    All lanes : Anti-SOCS2 antibody (ab66733) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 whole cell lysate (ab3957)
    Lane 4 : Raji whole cell lysate (ab30124)
    Lane 5 : Mouse thymus tissue lysate - total protein (ab29285)
    Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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