Mouse RANKL ELISA Kit (TRANCE/TNFSF11) (ab269553)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.85 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse RANKL ELISA Kit (TRANCE/TNFSF11)
See all RANKL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.94% Inter-assay Sample n Mean SD CV% Serum 3 3.24% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.85 pg/ml -
Range
6.25 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 103 102% - 104% Serum 105 105% - 107% EDTA Plasma 98 97% - 101% Cit plasma 97 96% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse RANKL ELISA Kit (TRANCE/TNFSF11) (ab269553) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RANKL (TRANCE/TNFSF11) protein in cell culture supernatant, edta plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse RANKL (TRANCE/TNFSF11) with 1.85 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Receptor activator of nuclear factor kappa-β ligand (RANKL) is a type II membrane protein and belongs to the tumor necrosis factor superfamily. RANKL is known to interact with TNFRS11B/OPG and TNFRSF11A/RANK which controls fundamental aspects of bone physiology and affects the immune system. Some of the specific functions of RANKL include essential regulator of bone remodeling, lymph node formation, and bone metastasis in cancer. ab269553 kit is developed to recognize extracellular soluble RANKL.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse RANKL (TRANCE/TNFSF11) Capture Antibody 1 x 600µl 10X Mouse RANKL (TRANCE/TNFSF11) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse RANKL (TRANCE/TNFSF11) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. -
Tissue specificity
Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. -
Involvement in disease
Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
Cellular localization
Cytoplasm; Secreted and Cell membrane. - Information by UniProt
-
Alternative names
- CD254
- hRANKL2
- ODF
see all -
Database links
- Entrez Gene: 21943 Mouse
- SwissProt: O35235 Mouse
- Unigene: 249221 Mouse
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Mouse RANKL standard curve comparisonStandard Curve comparison between Mouse RANKL SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
-
Example of mouse RANKL standard curve in Sample Diluent NS.The RANKL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native RANKL in mouse serum and plasma samples.The concentrations of RANKL were measured in duplicates, interpolated from the RANKL standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma-citate neat, and plasma-EDTA neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANKL concentration was determined to be 22.7 pg/mL in serum, and 18.3 pg/mL in plasma-citrate, and 22.3 pg/mL in plasma-EDTA.
-
Interpolated concentrations of native RANKL in mouse cell culture supernatant samples.The concentrations of RANKL were measured in duplicates, interpolated from the RANKL standard curves and corrected for sample dilution. Undiluted samples are as follows: neat stimulated splenocytes supernatant and neat unstimulated splenocytes supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANKL concentration was determined to be 28.5 pg/mL in stimulated splenocytes and 14.1 pg/mL in unstimulated splenocytes.
-
Sandwich ELISA - Mouse RANKL ELISA Kit (TRANCE/TNFSF11) (ab269553)To learn more about the advantages of recombinant antibodies see here.
