Anti-Hsp60 antibody (ab53109)
Key features and details
- Rabbit polyclonal to Hsp60
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp60 antibody
See all Hsp60 primary antibodies -
Description
Rabbit polyclonal to Hsp60 -
Host species
Rabbit -
Specificity
ab53109 detects endogenous levels of total Hsp60 protein. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Hsp60 aa 511-560.
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Positive control
- Human lung carcinoma tissue and extracts from COLO205 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hsp60 antibody (ab53109)All lanes : Anti-Hsp60 antibody (ab53109) at 1/300 dilution
Lane 1 : extract from COLO205 cells
Lane 2 : extract from COLO205 cells with immunising peptide
Predicted band size: 60 kDa
Observed band size: 60 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab53109)ab53109 at 1/50 dilution staining Hsp60 in human lung carcinoma tissue by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody (ab53109)ICC/IF image of ab53109 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53109, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
