Anti-p95/NBS1 (phospho S278) antibody (ab111373)
Key features and details
- Rabbit polyclonal to p95/NBS1 (phospho S278)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-p95/NBS1 (phospho S278) antibody
See all p95/NBS1 primary antibodies -
Description
Rabbit polyclonal to p95/NBS1 (phospho S278) -
Host species
Rabbit -
Specificity
ab111373 detects endogenous levels of p95/NBS1 only when phosphorylated at serine 278.
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB Human
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Immunogen
Synthetic peptide corresponding to Human p95/NBS1.
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Positive control
- HUVEC cell extracts, treated with foskolin; NIH3T3 cells.
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General notes
This product was previously labelled as p95 NBS1
Properties
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Form
Liquid -
Storage instructions
Frozen Stock (-20C). Shelf life 12 months. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab111373 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p95/NBS1 (phospho S278) antibody (ab111373)All lanes : Anti-p95/NBS1 (phospho S278) antibody (ab111373) at 1/500 dilution
Lane 1 : HUVEC cell extracts, treated with Forskolin (40nM, 30mins)
Lane 2 : HUVEC cell extracts, treated with Forskolin (40nM, 30mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 84 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 (phospho S278) antibody (ab111373)ab111373 at 1/100 dilution staining p95/NBS1 in NIH3T3 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.
