Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p95/NBS1 antibody [7E4C2] (ab181780)

Key features and details

  • Mouse monoclonal [7E4C2] to p95/NBS1
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-p95/NBS1 antibody [7E4C2]
    See all p95/NBS1 primary antibodies

  • Description

    Mouse monoclonal [7E4C2] to p95/NBS1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human p95/NBS1 aa 467-615. Expressed in E. Coli.
    Sequence:

    ERDEENQEMSSCKSARIETSCSLLEQTQPATPSLWKNKEQHLSENEPVDT NSDNNLFTDTDLKSIVKNSASKSHAAEKLRSNKKREMDDVAIEDEVLEQL FKDTKPELEIDVKVQKQEEDVNVRKRPRMDIETNDTFSDEAVPESSKIS


    Database link: O60934
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human p95/NBS1 recombinant protein; HEK293 cell lysate, transfected with p95/NBS1 (amino acids 467-615)-IgGFc; Jurkat cell lysate; HeLa cells; Human cervical cancer and colon cancer tissues.

  • General notes

     This product was previously labelled as p95 NBS1

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-p95/NBS1 antibody [7E4C2] (ab181780)
    Western blot - Anti-p95/NBS1 antibody [7E4C2] (ab181780)
    Anti-p95/NBS1 antibody [7E4C2] (ab181780) at 1/500 dilution + Human p95/NBS1 recombinant protein (amino acids 467-615)

    Predicted band size: 85 kDa

  • Western blot - Anti-p95/NBS1 antibody [7E4C2] (ab181780)
    Western blot - Anti-p95/NBS1 antibody [7E4C2] (ab181780)
    All lanes : Anti-p95/NBS1 antibody [7E4C2] (ab181780) at 1/500 dilution

    Lane 1 : HEK293 cell lysate, non-transfected
    Lane 2 : HEK293 cell lysate, transfected with p95/NBS1 (amino acids 467-615)-hIgGFc

    Predicted band size: 85 kDa

  • Western blot - Anti-p95/NBS1 antibody [7E4C2] (ab181780)
    Western blot - Anti-p95/NBS1 antibody [7E4C2] (ab181780)
    Anti-p95/NBS1 antibody [7E4C2] (ab181780) at 1/500 dilution + Jurkat cell lysate

    Predicted band size: 85 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 antibody [7E4C2] (ab181780)
    Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 antibody [7E4C2] (ab181780)

    Immunofluorescent analysis of HeLa cells labeling p95/NBS1 with ab181780 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Flow Cytometry - Anti-p95/NBS1 antibody [7E4C2] (ab181780)
    Flow Cytometry - Anti-p95/NBS1 antibody [7E4C2] (ab181780)

    Flow cytometric analysis of HeLa cells labeling p95/NBS1 with ab181780 at 1/200 dilution (green); negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4C2] (ab181780)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4C2] (ab181780)

    Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling p95/NBS1 with ab181780 at 1/200 dilution followed by DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4C2] (ab181780)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [7E4C2] (ab181780)

    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling p95/NBS1 with ab181780 at 1/200 dilution followed by DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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