Anti-GRK2 antibody - C-terminal (ab229669)
Key features and details
- Rabbit polyclonal to GRK2 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRK2 antibody - C-terminal
See all GRK2 primary antibodies -
Description
Rabbit polyclonal to GRK2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Synthetic peptide within Human GRK2 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated
Database link: P25098 -
Positive control
- WB: Jurkat, Raji and NCI-H929 whole cell lysate. IP: Jurkat whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-GRK2 antibody - C-terminal (ab229669)
GRK2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts with 5 μg ab229669. Western blot was then performed from the immunoprecipitate using ab229669. Anti-Rabbit IgG was used as a secondary antibody.
Lane 1: Jurkat whole cell extract (input)
Lane 2: Control IgG instead of ab229669 in Jurkat whole cell extract.
Lane 3: ab229669 IP in Jurkat whole cell extract. -
Western blot - Anti-GRK2 antibody - C-terminal (ab229669)All lanes : Anti-GRK2 antibody - C-terminal (ab229669) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 80 kDa7.5% SDS-PAGE gel.
