Anti-Keap1 antibody (ab118285) at 0.2 µg/ml + NIH3T3 cell lysate (in RIPA buffer) at 35 µg

Developed using the ECL technique.

Predicted band size: 70 kDa

ab118285 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing nuclear and cytoplasmic staining. The nuclear stained with DAPI.

ab118285 (3.8µg/ml) staining of paraffin embedded Human Cerebellum following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows textured cytoplasm staining in Purkinje cell and processes.