Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Wednesday March 17, 2021

Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [CPT-R35.3-41] to Thyroglobulin
Suitable for: WB, ELISA
Reacts with: Human

Product name

Anti-Thyroglobulin antibody [CPT-R35.3-41]
See all Thyroglobulin primary antibodies
Description

Rabbit monoclonal [CPT-R35.3-41] to Thyroglobulin
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human thyroid tissue lysate. ELISA: Immunizing peptide.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CPT-R35.3-41
Isotype

IgG
Research areas

ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)

50ng CPTAC-39d (1000ng/mL, 50 μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000 ng/mL) of ab243094 (50μL) were incubated with CPTAC-39d in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50 μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.

Secondary antibody was used at 1/2500 dilution.
Western blot - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)

All lanes : Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094) at 1/1000 dilution

Lane 1 : Human kidney tissue lysate
Lane 2 : Human thyroid tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 305 kDa
Observed band size: 330 kDa
why is the actual band size different from the predicted?

Exposure time: 37 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

Thyroglobulin is a glycoprotein synthesised exclusively by the thyroid follicular cells. The observed molecular weight is consistent with the reported MW (PMID: 14657389).

Negative control: Human kidney.
Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)

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