Exendin-4 ELISA Kit (ab272192)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.58 pg/ml
- Range: 11.72 pg/ml - 750 pg/ml
- Sample type: Cell culture media
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
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Product name
Exendin-4 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 6.1% Inter-assay Sample n Mean SD CV% Supernatant 3 1.9% -
Sample type
Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
2.58 pg/ml -
Range
11.72 pg/ml - 750 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture media 115 113% - 118% -
Assay time
1h 30m -
Assay duration
One step assay -
Product overview
Exendin-4 ELISA kit (ab272192) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Exendin-4 protein in cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Exendin-4 with 2.58 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Exendin-4 is a 39 amino acid peptide found in venom from the Gila monster Helicoderma suspectum. Exendin-4 is a member of the glucagon secretin family of peptide hormones and neuropeptides. The venom protein mimics the incretin hormone glucagon-like peptide 1 (GLP-1) and stimulates insulin synthesis and secretion, protects against beta-cell apoptosis in response to different insults, and promotes beta-cell proliferation. Exendin-4 also promotes satiety, reduces food intake, fat deposition and body weight. It also is known to inhibit gastric emptying. Exendin-4 interactions with GLP-1 receptor (GLP1R). induces hypotension that is mediated by relaxation of cardiac smooth muscle.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Exendin-4 Capture Antibody 1 x 600µl 10X Exendin-4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Exendin-4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Exendin 4 is a 39 amino acid peptide found in venom from the Gila monster Helicoderma suspectum. It is a member of the glucagon secretin family of peptide hormones and neuropeptides. Exendin 4 is a potent agonist of the GLP1 receptor and hence a potent stimulator of insulin secretion. -
Cellular localization
Secreted -
Alternative names
- Exenatide
- Exendin4
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of cell culture media Exendin-4 standard curve in Sample Diluent NS.The Exendin-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked* Exendin-4 RPMI-1640 cell culture media +/- 10% FBS samples.Interpolated concentrations of spiked* Exendin-4 RPMI-1640 cell culture media +/- 10% FBS samples.