Human Calcitonin ELISA Kit (ab279411)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.6 pg/ml
- Range: 4.7 pg/ml - 300 pg/ml
- Sample type: Cell culture media, EDTA Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Calcitonin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Urine 8 4.9% Inter-assay Sample n Mean SD CV% Urine 4 8.3% -
Sample type
Urine, Serum, Cell culture media, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.6 pg/ml -
Range
4.7 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 103 99% - 107% Serum 84 80% - 88% Cell culture media 94 91% - 96% EDTA Plasma 85 83% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Calcitonin ELISA kit (ab279411) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Calcitonin protein in human serum, plasma, urine, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Calcitonin with 1.6 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Calcitonin is a 32- amino acid long peptide hormone that is proteolytically cleaved from a larger prepropeptide and is encoded by the CALCA gene. Calcitonin causes a rapid, short-lived reduction in the concentration of calcium and phosphate in blood by the incorporation of the ions into bone. Secretion of calcitonin by the C-cells of the thyroid gland is stimulated by an increase in calcium serum levels, as well as the gastrointestinal hormones, gastrin and pentagastrin. Calcitonin inhibits both osteoclast activity in bones and renal tubular cell reabsorption of calcium and phosphate.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Calcitonin Capture Antibody 1 x 600µl 10X Human Calcitonin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Calcitonin Lyophilized Peptide Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity. -
Cellular localization
Cytoplasmic and Secreted -
Alternative names
- Alpha CGRP
- alpha type CGRP
- CALC 1
see all -
Database links
- Entrez Gene: 796 Human
- Omim: 114130 Human
- SwissProt: P01258 Human
- Unigene: 37058 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of human Calcitonin standard curve in Sample Diluent NS.The Calcitonin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Calcitonin in human urine.The concentrations of Calcitonin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 323 pg/mL in serum.