Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3359(2)] to Somatostatin 28 - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free
    See all Somatostatin 28 primary antibodies

  • Description

    Rabbit monoclonal [EPR3359(2)] to Somatostatin 28 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human pancreas tissue.

  • General notes

    Ab227422 is the carrier-free version of ab111912. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab227422 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)
    Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)

    Ab111912 staining Somatostatin-28 in Rat hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).  Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining in the hilus of the dentate gyrus of rat hippocampus [PMID: 25469233] . 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111912).

  • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)
    Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)

    Ab111912 staining Somatostatin-28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody.Counterstained with DAPI. Cytoplasmic staining on delta cells of rat pancreas islet. [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] .  

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111912).

  • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)
    Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)

    Ab111912 staining Somatostatin-28 in Mouse hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100.Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody.Counterstained with DAPI.  Cytoplasmic staining in the hilus of the dentate gyrus of mouse hippocampus [PMID: 25469233]. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111912).

  • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)
    Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)

    Ab111912 staining Somatostatin-28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining on delta cells of mouse pancreas islet. [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111912).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)

    ab111912 staining Somatostatin 28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediated in a TRIS/EDTA buffer pH9 (ab93684).Primary antibody at 1:4000 dilution and Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of rat pancreas islet[PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32]  .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111912).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)

    ab111912 staining Somatostatin 28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (ab93684). Primary antibody at 1:4000 dilution and   a  Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Couterstained with hematoxylin. Cytoplasmic staining on delta cells of mouse pancreas islet [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111912).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)

    ab111912 staining Somatostatin 28 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (ab93684). Primary antibody  at 1/4000 dilution and a Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of human pancreas islet. [PMID: 25019573], [PMID: 9066918],  [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111912).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)

    ab111912, at a 1/50 dilution, staining Somatostatin 28 in paraffin embedded Human pancreas tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111912).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)
    Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)

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