Anti-beta II Tubulin antibody [EPR16773] - BSA and Azide free (ab250105)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16773] to beta II Tubulin - BSA and Azide free
- Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-beta II Tubulin antibody [EPR16773] - BSA and Azide free
See all beta II Tubulin primary antibodies -
Description
Rabbit monoclonal [EPR16773] to beta II Tubulin - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab250105 is the carrier-free version of ab179512. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250105 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16773 -
Isotype
IgG -
Research areas
Images
-
Anti-beta II Tubulin antibody [EPR16773] (ab179512) at 1/10000 dilution + Human fetal brain lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaThis data was developed using ab179512, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Beta II Tubulin is highly expressed in brain tissues and cell lines derived from the central nervous system.
-
All lanes : Anti-beta II Tubulin antibody [EPR16773] (ab179512) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaThis data was developed using ab179512, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Beta II Tubulin is highly expressed in brain tissues and cell lines derived from the central nervous system.
-
All lanes : Anti-beta II Tubulin antibody [EPR16773] (ab179512) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : C6 (Rat glial tumor cells) whole cell lysate
Lane 9 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaThis data was developed using ab179512, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Beta II Tubulin is highly expressed in brain tissues and cell lines derived from the central nervous system.
-
This data was developed using ab179512, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling beta II Tubulin with ab179512 at 1/100 dilution, followed by anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/400 dilution (green). Confocal image shows both cytoplasm and nuclear staining on the SH-SY5Y cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: - ab179512 at 1/100 dilution followed by anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.
-ve control 2:. - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by anti-rabbit Alexa Fluor® 488 (ab150077) at 1/400 dilution. -
This data was developed using ab179512, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. High level of cytoplasmic and nuclear expression on neuron cells of Human cerebral cortex is observed. Counter stained with Hematoxylin. Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab179512, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human glioma labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on Human glioma cells is observed. Counter stained with Hematoxylin. Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
This data was developed using ab179512, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. High level of expression on neuron cells of Mouse cerebral cortex is observed. Counter stained with Hematoxylin. Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
This data was developed using ab179512, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. High level of expression on neuron cells of Rat cerebral cortex is observed. Counter stained with Hematoxylin. Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
This data was developed using ab179512, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling beta II Tubulin with ab179512 at 1/450 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
-
This data was developed using ab179512, the same antibody clone in a different buffer formulation.
Beta II Tubulin was immunoprecipitated from 1mg of Human fetal brain extract with ab179512 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab179512 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal brain extract. Lane 2: PBS instead of extract.
Blocking and dilution buffer: 5% NFDM/TBST.
-