Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PHLDA1 antibody [EPR6674] (ab133654)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6674] to PHLDA1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-PHLDA1 antibody [EPR6674]
    See all PHLDA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6674] to PHLDA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region within Human PHLDA1 (UniProt ID: Q8WV24).

  • Positive control

    • Human melanoma tissue lysate; A375 and BxPC3 cell lysates; Human colon carcinoma tissue; BxPC3 cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PHLDA1 antibody [EPR6674] (ab133654)
    Western blot - Anti-PHLDA1 antibody [EPR6674] (ab133654)
    All lanes : Anti-PHLDA1 antibody [EPR6674] (ab133654) at 1/10000 dilution

    Lane 1 : Human melanoma tissue lysate
    Lane 2 : A375 cell lysate
    Lane 3 : BxPC3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PHLDA1 antibody [EPR6674] (ab133654)
    Immunocytochemistry/ Immunofluorescence - Anti-PHLDA1 antibody [EPR6674] (ab133654)
    Immunocytochemical analysis of PHLDA1 in BxPC3 cells labelled with ab133654 at a 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDA1 antibody [EPR6674] (ab133654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDA1 antibody [EPR6674] (ab133654)

    Immunohistochemical analysis of PHLDA1 in paraffin embedded Human colon carcinoma tissue labelled with ab133654 at a 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-PHLDA1 antibody [EPR6674] (ab133654)
    OI-RD Scanning - Anti-PHLDA1 antibody [EPR6674] (ab133654)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PHLDA1 antibody [EPR6674] (ab133654)
    Anti-PHLDA1 antibody [EPR6674] (ab133654)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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