Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human PDGF CC protein (ab84066)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human PDGF CC protein

  • Biological activity

    Biological Activity : Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 15-20 ng/ml.

  • Purity

    > 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MVVDLNLLTE EVRLYSCTPR NFSVSIREEL KRTDTIFWPG CLLVKRCGGN CACCLHNCNE CQCVPSKVTK KYHEVLQLRP KTGVRGLHKS LTDVALEHHE ECDCVCRGST GG

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab84066 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        SDS-PAGE

        Functional Studies

      • Form

        Lyophilized

      • Additional notes

        Endotoxin level is less than 0.1 ng per µg (1EU/µg).
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Reconstituted PDGF CC is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

      General Info

      • Alternative names

        • FALLOTEIN
        • PDGF C
        • PDGFC
        • Platelet derived growth factor
        • platelet-derived growth factor C
        • SCDGF
        • secretory growth factor-like protein
        • Spinal cord-derived growth factor
        • VEGF E
        see all

      • Relevance

        The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14 kDa polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-alpha and PDGFR-beta, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet alpha-granules and are released upon platelet activation. PDGF-AA, -AB, -BB and –CC signal primarily through the PDGF R alpha receptor, whereas PDGF-DD interacts almost exclusively with the PDGF R beta receptor.

      • Cellular localization

        Cytoplasmic, Nuclear, Secreted and Cytoplasmic granule

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab84066? Please let us know so that we can cite the reference in this datasheet.

    ab84066 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • FALLOTEIN
      • PDGF C
      • PDGFC
      • Platelet derived growth factor
      • platelet-derived growth factor C
      • SCDGF
      • secretory growth factor-like protein
      • Spinal cord-derived growth factor
      • VEGF E
      see all

    • Relevance

      The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14 kDa polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-alpha and PDGFR-beta, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet alpha-granules and are released upon platelet activation. PDGF-AA, -AB, -BB and –CC signal primarily through the PDGF R alpha receptor, whereas PDGF-DD interacts almost exclusively with the PDGF R beta receptor.

    • Cellular localization

      Cytoplasmic, Nuclear, Secreted and Cytoplasmic granule

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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