Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)
![Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)](https://www.abcam.com/ps/products/203/ab203264/Images/ab203264-247514-anti-eb3-antibody-epr11421b-alexa-fluor-488-immunofluorescence.jpg "Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR11421(B)] to EB3
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)]
See all EB3 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR11421(B)] to EB3 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Mouse
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Neuro2a cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11421(B) -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)
ab203264 staining EB3 in Neuro2a cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203264 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)](https://www.abcam.com/ps/products/203/ab203264/Images/ab203264-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-EB3 antibody [EPR11421(B)] (ab203264)
