Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EB3 antibody [KT36] (ab53360)

Key features and details

  • Rat monoclonal [KT36] to EB3
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Human, African green monkey
  • Isotype: IgG2a

Overview

  • Product name

    Anti-EB3 antibody [KT36]
    See all EB3 primary antibodies

  • Description

    Rat monoclonal [KT36] to EB3

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    African green monkey
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length human EB3 fused to a tag, purified from E. coli BL21 bacteria.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-EB3 antibody [KT36] (ab53360)
    Western blot - Anti-EB3 antibody [KT36] (ab53360)
    Anti-EB3 antibody [KT36] (ab53360) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 8 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-EB3 antibody [KT36] (ab53360)
    Immunocytochemistry/ Immunofluorescence - Anti-EB3 antibody [KT36] (ab53360) This image is courtesy of Dr Anna Akhmanova
    Immunofluorescent staining for COS7 cells overexpressing EB3, using ab53360 at 1/100.
  • Immunocytochemistry/ Immunofluorescence - Anti-EB3 antibody [KT36] (ab53360)
    Immunocytochemistry/ Immunofluorescence - Anti-EB3 antibody [KT36] (ab53360) This image is courtesy of Dr Anna Akhmanova
    Immunofluorescent staining of cell mitosis using ab53360 crude culture supernatant diluted 1:10 (Please note that there was about 50-100 ug/ml antibody in the supernatants) COS7 Transformed African Green Monkey Kidney Fibroblast Cells.
  • Flow Cytometry - Anti-EB3 antibody [KT36] (ab53360)
    Flow Cytometry - Anti-EB3 antibody [KT36] (ab53360)
    Overlay histogram showing SH-SY5Y cells stained with ab53360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53360, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-EB3 antibody [KT36] (ab53360)

© 2021 Astana Biomed Group LLP. All rights reserved.